ABSTRACT
Abstract INTRODUCTION Peripheral blood of 400 dogs infected with Leishmania and Ehrlichia were analyzed using polymerase chain reaction (PCR), and clinical signs were characterized. METHODS PCR and parasitological tests were conducted. RESULTS PCR was positive for Leishmania in 84.75%, and parasitological tests showed that 63.25% and 31.75% were positive for Leishmania and Ehrlichia, respectively. All animals showed more than three clinical signs. PCR results were negative for Leishmania in 15.25% of the samples. CONCLUSIONS Conventional PCR of peripheral blood can be used for diagnosing canine visceral leishmaniasis in combination with other techniques, especially in uncertain cases that need species identification.
Subject(s)
Animals , Male , Female , Dogs , Ehrlichiosis/veterinary , Dog Diseases/diagnosis , Leishmaniasis, Visceral/veterinary , Brazil/epidemiology , Polymerase Chain Reaction , Sensitivity and Specificity , Ehrlichiosis/diagnosis , Ehrlichiosis/epidemiology , Endemic Diseases , Dog Diseases/epidemiology , Coinfection , Leishmaniasis, Visceral/diagnosis , Leishmaniasis, Visceral/epidemiologyABSTRACT
This work aimed to evaluate the toxicity of some insecticides compounds on Aedes aegypti and Artemia salina larvae. Bioassays were carried out to evaluate the toxic effect after of 24 and 72 h using the compounds or associations. The LC10, LC50 and LC90 values were obtained and utilized for toxicity comparations. For Ae. aegypti, LC50 were 32.65 mg L-1 in 24 h for Na2[EDTA-Cu(II)] and total mortality in 72 h for SAP-Na2[EDTA-Cu(II)].
ABSTRACT
This work aimed to evaluate the toxicity of some insecticides compounds on Aedes aegypti and Artemia salina larvae. Bioassays were carried out to evaluate the toxic effect after of 24 and 72 h using the compounds or associations. The LC10, LC50 and LC90 values were obtained and utilized for toxicity comparations. For Ae. aegypti, LC50 were 32.65 mg L-1 in 24 h for Na2[EDTA-Cu(II)] and total mortality in 72 h for SAP-Na2[EDTA-Cu(II)].
Aedes aegypti é inseto de grande interesse na Saúde Pública por transmitir o vírus da Dengue, Febre Amarela e Febre Hemorrágica da Dengue. No controle do mosquito utilizam-se inseticidas organoclorados, organofosforados, carbamatos e piretróides que guiam à seleção de insetos resistentes. A combinação de íons metálicos de transição, EDTA, STI e SAP são propostos como estratégia efetiva e persistente para o controle de larvas de Ae. aegypti através de danos ao sistema digestório por radicais livres. A toxicidade dos compostos foi avaliada para Ae. aegypti e A. salina (organismo não alvo). Os bioensaios foram conduzidos para avaliar o efeito tóxico dos compostos e associações após 24 e 72 horas. As CL10, CL50 e CL90 foram obtidas para comparações de toxicidade. Para Ae. aegypti a CL50 foi 32,65 mg L-1 em 24 horas para Na2[EDTA-Cu(II)] e mortalidade total a 62,5 mg.L-1para quelato em polímero acrílico de liberação lenta SAP-Na2[EDTA-Cu(II)] em até 72 horas.